Composite
Part:BBa_K3924047:Design
Designed by: Wenda Zhang Group: iGEM21_Tsinghua (2021-10-21)
mchE-mchF polycistrons with Ptac lacO promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1713
Illegal PstI site found at 610
Illegal PstI site found at 823 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1713
Illegal PstI site found at 610
Illegal PstI site found at 823 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1713
Illegal BamHI site found at 237
Illegal BamHI site found at 2380 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1713
Illegal PstI site found at 610
Illegal PstI site found at 823 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1713
Illegal PstI site found at 610
Illegal PstI site found at 823
Illegal AgeI site found at 2761 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1347
Illegal SapI site found at 2456
Design Notes
mchE-mchF polycistrons with Ptac lacO promoter
Source
mchE-mchF polycistrons with Ptac lacO promoter